Roger Helmer has tentatively threatened Conservative Leader David Cameron, saying he would "personally regard our commitment not to accept the Lisbon Treaty as the heart of the Manifesto on which I was elected two weeks ago, and I should regard any retreat from it as a betrayal of our supporters, and of the British people."

The Conservative MEP for the East Midlands made the threat on his blog. He went on to say that he would have to have to consider his position in the Party "very carefully" if Cameron did not give the British people a referendum on the Lisbon Treaty, even if it is ratified by the Irish second time round.

Helmer's comments come after Conservatives Shadow Business Secretary Ken Clarke seemingly announced that the Tories would not grant a referendum if the Treaty was ratified by the time a Conservative government was in place.

The Conservative Party's former chief donor Stuart Wheeler is to speak at a Better Off Out (of the EU) meeting organised by The Freedom Association.

Stuart will join Roger Helmer MEP, Lindsay Jenkins, the Rev'd Dr. Peter Mullen and Sam Westrop on the platform at the Better Off Out event in Gloucestershire.

Wheeler made headlines earlier this year when he was expelled from the Conservative Party for announcing he would vote UKIP in the European Election and donated £100k to the Party. In 2001, Wheeler gave the Conservatives their largest ever donation of £5 million.

More details of the meeting can be found at the Better Off Out website.

The Europe of Freedom and Democracy group, comprising 30 MEPs from eight countries, includes UKIP who have 13 MEPs and Italy's Lega Nord who have 9 MEPs and are in coalition government with Silvio Berlusconi.

Lega Nord were in the UEN group in the last parliament but have now joined forces with UKIP. Like UKIP, Lega Nord were successful in the European Election, doubling the number of seats it won from four to eight and winning 9.5% of the vote in Italy compared with 5% in the 2004 elections.

Leadership of the new group will be split between UKIP leader Nigel Farage and Frencesco Speroni from Lega Nord.

Speaking at the group's first meeting, Farage also confirmed the group will not give its support to Jose Manuel Barroso in his attempts to win a second term as commission president. Farage admitted that the "campaign to unseat" Barroso was still likely to end in failure.

With regards to the new group, Farage said it would provide a "much-needed voice of opposition" in the Euro Parliament, just as the Independence and Democracy group had done in the 2004 - 2009 Parliament. He said it was striking that in the last parliament the EPP and Socialists, parliament's two biggest groups, had voted together on major policy issues on 97 per cent of occasions. "We are going to try and fight this cosy consensus," he said. "I am confident we can make a difference. If we don't then who will?"

Other comments came from other members of the group, including Morten Messerschmidt, of the Danish People's Party, who said the group would "speak out against further EU integration."The group also includes Philippe de Villiers, elected on a Libertas ticket, and former EU "whistleblower", UKIP MEP Marta Andreasen. She said the group would propose her for a deputy chairmanship of the key budgetary control committee, adding, "It is an important post and one where I could make a real impact."

The group has two MEPs from Denmark and two from Greece, one each from Finland, France, Slovakia and the Netherlands as well as the UK and Italian members.